Please use this identifier to cite or link to this item: https://repositorio.uca.edu.ar/handle/123456789/9493
Título : In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal
Autor : Sansiñena, Marina Julia 
Santos, María Victoria 
Chirife, Jorge 
Zaritzky, Noemí 
Palabras clave : FERTILIZACION IN VITROTRANSFERENCIA DE CALORDESARROLLO EMBRIONARIOCRIOPRESERVACIONVITRIFICACIONOVOCITOSBOVINOS
Fecha de publicación : 2018
Editorial : Elsevier
Cita : Sansiñena,M.J. et. al. In-vitro development of vitrified-warmed bovine oocytes after activation may be predicted based on mathematical modelling of cooling and warming rates during vitrification, storage and sample removal [en línea]. Reproductive BioMedicine. 2018, 36(5). doi:10.1016/j.rbmo.2018.01.003 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9493
Resumen : Abstract: Heat transfer during cooling and warming is difficult to measure in cryo-devices; mathematical modelling is an alternative method that can describe these processes. In this study, we tested the validity of one such model by assessing in-vitro development of vitrified and warmed bovine oocytes after parthenogenetic activation and culture. The viability of oocytes vitrified in four different cryo-devices was assessed. Consistent with modelling predictions, oocytes vitrified using cryo-devices with the highest modelled cooling rates had significantly (P < 0.05) better cleavage and blastocyst formation rates. We then evaluated a two-step sample removal process, in which oocytes were held in nitrogen vapour for 15 s to simulate sample identification during clinical application, before being removed completely and warmed. Oocytes exposed to this procedure showed reduced developmental potential, according to the model, owing to thermodynamic instability and devitrification at relatively low temperatures. These findings suggest that cryo-device selection and handling, including method of removal from nitrogen storage, are critical to survival of vitrified oocytes. Limitations of the study include use of parthenogenetically activated rather than fertilized ova and lack of physical measurement of recrystallization. We suggest mathematical modelling could be used to predict the effect of critical steps in cryopreservation.
URI : https://repositorio.uca.edu.ar/handle/123456789/9493
ISSN : 1472-6483 (impreso)
1472-6491 (online)
Disciplina: CIENCIAS AGRARIAS
DOI: 10.1016/j.rbmo.2018.01.003
Derechos: Acceso abierto
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