Please use this identifier to cite or link to this item: https://repositorio.uca.edu.ar/handle/123456789/5491
Título : How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor?
Autor : Santos, María Victoria 
Sansiñena, Marina Julia 
Zaritzky, Noemí 
Chirife, Jorge 
Palabras clave : CONGELACIONESPERMA BOVINOTRANSFERENCIA DE CALORVAPOR DE NITROGENOCOEFICIENTE
Fecha de publicación : 2013
Editorial : Ingenta
Cita : Santos, M. V., Sansinena, M., Zaritzky, N. y J. Chirife. 2013. How important are internal temperature gradients in french straws during freezing of bovine sperm in nitrogen vapor? [en línea]. CryoLetters 34 (2). Disponible en: https://repositorio.uca.edu.ar/handle/123456789/5491
Resumen : Abstract: The subject of present work was to predict internal temperature gradients developed during freezing of bovine sperm diluted in extender, packaged in 0.5 ml French plastic straws and suspended in static liquid nitrogen vapor at -100 ºC. For this purpose, a mathematical heat transfer model previously developed to predict freezing times (phase change was considered) of semen/extender packaged in straw was extended to predict internal temperature gradients during the cooling/freezing process. Results showed maximum temperature differences between the centre and the periphery of semen/extender “liquid” column was 1.5 ºC for an external heat transfer coefficient, h = 15 W/(m2 K), and only 0.5 ºC for h = 5 W/(m2 K). It is concluded that if a thermocouple wire were inserted in a 0.5 ml plastic straw to monitor the freezing process in nitrogen vapor, its radial position would have little importance since expected internal gradients may be safely neglected. This finding facilitates the interpretation of freezing rates in 0.5 ml plastic straws immersed in nitrogen vapor over liquid nitrogen, a widely used method for cryopreservation of bovine spermatozoa
URI : https://repositorio.uca.edu.ar/handle/123456789/5491
ISSN : 0143-2044
1742-0644 (Online)
Disciplina: PRODUCCION AGROPECUARIA
Derechos: Acceso Abierto
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