Please use this identifier to cite or link to this item: https://repositorio.uca.edu.ar/handle/123456789/5463
Título : Fluctuating temperatures terminate dormancy in cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA synthesis or signalling
Autor : Huarte, Héctor Roberto 
Luna, Virginia 
Pagano, Eduardo A. 
Zavala, Jorge A. 
Benech Amold, Roberto L. 
Otros colaboradores: Universidad Católica Argentina (Buenos Aires). Facultad de Ciencias Agrarias
Universidad Nacional de Río Cuarto (Argentina). Departamento de Ciencias Naturales. Laboratorio de Fisiología Vegetal
Universidad de Buenos Aires (Argentina). Facultad de Agronomía
Consejo Nacional de Investigaciones Científicas y Técnicas. CONICET (Argentina)
Universidad de Buenos Aires ; Conicet (Argentina). Instituto de Investigaciones Fisiológicas y Ecológicas vinculadas a la Agricultura
Palabras clave : ACIDO ABSCISICOINACTIVIDADTEMPERATURAEXPRESION GENICAGIBERELINAS
Fecha de publicación : 2014
Cita : Huarte, H. R., Luna, V., Pagano, E. A., Zavala, J. A. y R. L. Benech Amold.. 2014. Fluctuating temperatures termminate dormancy in cynara cardunculus seeds by turning off ABA synthesis and reducing ABA signalling, but not stimulating GA sunthesis or signalling [en línea]. Seed Science Research 24. Disponible en: doi:10.1017/S0960258514000051 Disponible en: http://bibliotecadigital.uca.edu.ar/repositorio/investigacion/fluctuating-temperatures-dormancy.pdf [Fecha de consulta: ….]
Resumen : Abstract: Fluctuating temperatures terminate seed dormancy in many species, including Cynara cardunculus (L.) var. sylvestris. Termination of physiological dormancy requires low ratios of abscisic acid (ABA)/gibberellins (GA). In a previous paper we have shown that physiological responses to fluctuating temperatures comprise a reduction of abscisic acid (ABA) content and sensitivity. However, a possible stimulation of GA synthesis was also suggested as part of the mechanism. That possible stimulation, as well as the identification of potential regulatory sites for ABA and GA metabolism and signalling involved in the termination of dormancy by fluctuating temperatures, are yet to be determined. In this study, we measured GA content and sensitivity in seeds incubated under constant and fluctuating temperatures. We also assessed the expression of several genes involved in ABA and GA metabolism and signalling. Our results show that fluctuating temperatures reduce ABA/GA ratios through a reduction in ABA accumulation during incubation but without altering GA synthesis as compared to that observed under constant temperatures. On the other hand, fluctuating temperatures did not increase sensitivity to GA. Fluctuating temperatures reduced the expression of CycaNCED and CycaABI5 (ABA synthesis and signalling genes) with a temporal pattern that coincides with the interruption of ABA accumulation that precedes germination of seeds incubated under fluctuating temperatures. However, fluctuating temperatures did not modify the expression of CycaCYP707A2 (ABA inactivation) as compared to that observed under constant temperatures. Consistent with our determinations of GA content and sensitivity, fluctuating temperatures did not modify the expression of GA synthesis (CycaGA3ox) and signalling genes (CycaRGL2 and CycaGAI) in relation to that observed at constant temperatures. These results show that fluctuating temperatures terminate dormancy in Cynara cardunculus seeds through an interruption in ABA accumulation and a reduction in ABA signalling exerted at the level of CycaNCED and CycaABI5 expression
URI : https://repositorio.uca.edu.ar/handle/123456789/5463
Disciplina: PRODUCCION AGROPECUARIA
Derechos: Acceso Abierto
Appears in Collections:Artículos

Files in This Item:
File Description SizeFormat
fluctuating-temperatures-dormancy.pdf201,73 kBAdobe PDFThumbnail
View/Open
Show full item record

Page view(s)

56
checked on Feb 27, 2021

Download(s)

40
checked on Feb 27, 2021

Google ScholarTM

Check



This item is licensed under a Creative Commons License Creative Commons