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Título : Mapping archaeal diversity in soda lakes by coupling 16S rRNA PCR-DGGE analysis with remote sensing and GIS technology
Autor : Elshafey, Naglaa 
Selim, Samy 
Mohammed, Asmaa H. 
Hagagy, Nashwa 
Samy, Mennatalla 
Mostafa, Ehab M. 
Safhi, Fatmah A. 
Saddiq, Amna 
Alsharari, Salam S. 
Aseel, Dalia G. 
Hafiz, Iram 
Elkelish, Amr 
Pérez, Leonardo Martín 
Palabras clave : HALOARQUEASBIOTECNOLOGIABIODIVERSIDAD
Fecha de publicación : 2022
Editorial : MDPI
Cita : Elshafey, N., et al. Mapping archaeal diversity in soda lakes by coupling 16S rRNA PCR-DGGE analysis with remote sensing and GIS technology [en línea]. Fermentation. 2022, 8(8), 365, doi:10.3390/fermentation8080365 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14809
Resumen : Abstract: The haloarchaeal diversity of four hypersaline alkaline lakes from the Wadi El-Natrun depression (Northern Egypt) was investigated using culture-independent polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene phylotypes, which was combined with remote sensing and geographic information system (GIS) data to highlight the distribution pattern of the microbial diversity in water and sediment samples. The majority of archaeal sequences identified in all four lakes belonged to the phyla Euryarchaeota and Crenarchaeota. Sediment samples from Beida Lake and water samples from El-Hamra Lake showed the highest levels of archaeal diversity. Sequence similarities ≥ 95% were found between six of the acquired clones and uncultured Halorhabdus, Euryarchaeota, and archaeon clones. In addition, two clones shared a high level of sequence similarity (97%) with unclassified archaea, while other nine clones exhibited 96% to 99% sequence similarity with uncultured archaeon clones, and only one clone showed 97% identity with an uncultured Crenarchaeota. Likewise, 7 DGGE bands presented a sequence similarity of 90 to 98% to Halogranum sp., Halalkalicoccus tibetensis, Halalkalicoccus jeotgali, uncultured Halorubrum, Halobacteriaceae sp., or uncultured haloarchaeon. In conclusion, while the variety of alkaliphilic haloarchaea in the examined soda lakes was restricted, the possibility of uncovering novel species for biotechnological applications from these extreme habitats remains promising.
URI : https://repositorio.uca.edu.ar/handle/123456789/14809
ISSN : 2311-5637
Disciplina: INGENIERIA AMBIENTAL
DOI: 10.3390/ fermentation8080365
Derechos: Acceso abierto
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