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https://repositorio.uca.edu.ar/handle/123456789/10232
Título : | Analysis of cholesterol in mouse brain by HPLC with UV detection | Autor : | Paulazo, Maria Alejandra Sodero, Alejandro Omar |
Palabras clave : | COLESTEROL; CEREBRO; CROMATOGRAFIA LIQUIDA | Fecha de publicación : | 2020 | Editorial : | Public Library of Science | Cita : | Paulazo, M. A., Sodero, A. O. Analysis of cholesterol in mouse brain by HPLC with UV detection [en línea]. PLoS ONE. 2020, 15(1): e0228170. doi:10.1371/journal.pone.0228170 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10232 | Resumen : | Abstract: We describe a sensitive high performance liquid chromatography (HPLC)-based method for the determination of cholesterol in brain tissue. The method does not require the derivatization of the analyte and uses separation and quantification by reversed-phase HPLC coupled to UV detection. Lipids were methanol/chloroform extracted following the method of Bligh and Dyer, and separated using isopropanol/acetonitrile/water (60/30/10, v/v/v) as mobile phase. We observed lineal detection in a wide range of concentrations, from 62.5 to 2000 ng/μL, and were able to detect a significant increase in the brain cholesterol levels between postnatal days 2 and 10 in C57BL6 mice. Based on our validation parameters, we consider this analytical method a useful tool to assess free cholesterol in rodent brain samples and cell cultures. | URI : | https://repositorio.uca.edu.ar/handle/123456789/10232 | ISSN : | 1932-6203 | Disciplina: | MEDICINA | DOI: | 10.1371/journal.pone.0228170 | Derechos: | Acceso abierto |
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