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Título : Analysis of cholesterol in mouse brain by HPLC with UV detection
Autor : Paulazo, Maria Alejandra 
Sodero, Alejandro Omar 
Fecha de publicación : 2020
Editorial : Public Library of Science
Cita : Paulazo, M. A., Sodero, A. O. Analysis of cholesterol in mouse brain by HPLC with UV detection [en línea]. PLoS ONE. 2020, 15(1): e0228170. doi:10.1371/journal.pone.0228170 Disponible en:
Resumen : Abstract: We describe a sensitive high performance liquid chromatography (HPLC)-based method for the determination of cholesterol in brain tissue. The method does not require the derivatization of the analyte and uses separation and quantification by reversed-phase HPLC coupled to UV detection. Lipids were methanol/chloroform extracted following the method of Bligh and Dyer, and separated using isopropanol/acetonitrile/water (60/30/10, v/v/v) as mobile phase. We observed lineal detection in a wide range of concentrations, from 62.5 to 2000 ng/μL, and were able to detect a significant increase in the brain cholesterol levels between postnatal days 2 and 10 in C57BL6 mice. Based on our validation parameters, we consider this analytical method a useful tool to assess free cholesterol in rodent brain samples and cell cultures.
ISSN : 1932-6203
Disciplina: MEDICINA
DOI: 10.1371/journal.pone.0228170
Derechos: Acceso abierto
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