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Título : Deep transcriptomic profiling of M1 macrophages lacking Trpc3
Autor : Kumarasamy, Sivarajan 
Solanki, Sumeet 
Atolagbe, Oluwatomisin T. 
Joe, Bina 
Birnbaumer, Lutz 
Vazquez, Guillermo 
Fecha de publicación : 2017
Editorial : Nature Research
Cita : Kumarasamy S, Solanki S, Atolagbe OT, Joe B, Birnbaumer L, Vazquez G. Deep transcriptomic profiling of M1 macrophages lacking Trpc3 [en línea]. Scientific Reports. 2017;7:39867. doi:10.1038/srep39867 Disponible en:
Resumen : Abstract: In previous studies using mice with macrophage-specific loss of TRPC3 we found a significant, selective effect of TRPC3 on the biology of M1, or inflammatory macrophages. Whereas activation of some components of the unfolded protein response and the pro-apoptotic mediators CamkII and Stat1 was impaired in Trpc3-deficient M1 cells, gathering insight about other molecular signatures within macrophages that might be affected by Trpc3 expression requires an alternative approach. In the present study we conducted RNA-seq analysis to interrogate the transcriptome of M1 macrophages derived from mice with macrophage-specific loss of TRPC3 and their littermate controls. We identified 160 significantly differentially expressed genes between the two groups, of which 62 were upregulated and 98 downregulated in control vs. Trpc3-deficient M1 macrophages. Gene ontology analysis revealed enrichment in processes associated to cellular movement and lipid signaling, whereas the enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways included networks for calcium signaling and cell adhesion molecules, among others. This is the first deep transcriptomic analysis of macrophages in the context of Trpc3 deficiency and the data presented constitutes a unique resource to further explore functions of TRPC3 in macrophage biology.
ISSN : 2045-2322
Disciplina: MEDICINA
DOI: 10.1038/srep39867
Derechos: Acceso Abierto
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