Por favor, use este identificador para citar o enlazar este ítem:
https://repositorio.uca.edu.ar/handle/123456789/8685
Título : | M1 macrophage polarization is dependent on TRPC1-mediated calcium entry | Autor : | Chauhan, Arun Sun, Yuyang Sukumaran, Pramod Quenum Zangbede, Fredice O. Jondle, Christopher N. Sharma, Atul Evans, Dustin L. Chauhan, Pooja Szlabick, Randolph E. Aaland, Mary O. Birnbaumer, Lutz Sharma, Jyotika Singh, Brij B. Mishra, Bibhuti B. |
Palabras clave : | INMUNOLOGIA; SISTEMA INMUNOLOGICO; CALCIO; SEPSIS | Fecha de publicación : | 2018 | Editorial : | Elsevier (Cell Press) | Cita : | Chauhan A, Sun Y, Sukumaran P, et al. M1 macrophage polarization is dependent on TRPC1-mediated calcium entry. iScience. 2018;8:85-102. doi:10.1016/j.isci.2018.09.014 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8685 | Resumen : | Abstract: Macrophage plasticity is essential for innate immunity, but in-depth signaling mechanism(s) regulating their functional phenotypes are ill-defined. Here we report that interferon (IFN) γ priming of naive macrophages induces store-mediated Ca2+ entry and inhibition of Ca2+ entry impairs polarization to M1 inflammatory phenotype. In vitro and in vivo functional analyses revealed ORAI1 to be a primary contributor to basal Ca2+ influx in macrophages, whereas IFNγ-induced Ca2+ influx was mediated by TRPC1. Deficiency of TRPC1 displayed abrogated IFNγ-induced M1 inflammatory mediators in macrophages. In a preclinical model of peritonitis by Klebsiella pneumoniae infection, macrophages showed increased Ca2+ influx, which was TRPC1 dependent. Macrophages from infected TRPC1-/- mice showed inhibited expression of M1-associated signature molecules. Furthermore, in human patients with systemic inflammatory response syndrome, the level of TRPC1 expression in circulating macrophages directly correlated with M1 inflammatory mediators. Overall, TRPC1-mediated Ca2+ influx is essential for the induction/shaping of macrophage polarization to M1 inflammatory phenotype. | URI : | https://repositorio.uca.edu.ar/handle/123456789/8685 | ISSN : | 2589-0042 | Disciplina: | MEDICINA | DOI: | 10.1016/j.isci.2018.09.014 | Derechos: | Acceso Abierto |
Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
m1-macrophage-polarization-dependent.pdf | 6,05 MB | Adobe PDF | Visualizar/Abrir |
Visualizaciones de página(s)
130
comprobado en 11-oct-2024
Descarga(s)
154
comprobado en 11-oct-2024
Google ScholarTM
Consultar
Altmetric
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons