Please use this identifier to cite or link to this item: https://repositorio.uca.edu.ar/handle/123456789/8684
Título : A mirror code for protein cholesterol interactions in the two leaflets of biological membranes
Autor : Fantini, Jacques 
Di Scala, Coralie 
Evans, Luke S. 
Williamson, Philip, T. F. 
Barrantes, Francisco José 
Palabras clave : MEDICINACOLESTEROLPROTEINASNEUROTRANSMISORES
Fecha de publicación : 2016
Editorial : Nature Research
Cita : Fantini, J. et al. A mirror code for protein-cholesterol interactions in the two leaflets of biological membranes. Sci. Rep. 6, 21907; doi: 10.1038/srep21907 (2016). Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8684
Resumen : Abstract: Cholesterol controls the activity of a wide range of membrane receptors through specific interactions and identifying cholesterol recognition motifs is therefore critical for understanding signaling receptor function. The membrane-spanning domains of the paradigm neurotransmitter receptor for acetylcholine (AChR) display a series of cholesterol consensus domains (referred to as “CARC”). Here we use a combination of molecular modeling, lipid monolayer/mutational approaches and NMR spectroscopy to study the binding of cholesterol to a synthetic CARC peptide. The CARC-cholesterol interaction is of high affinity, lipid-specific, concentration-dependent, and sensitive to single-point mutations. The CARC motif is generally located in the outer membrane leaflet and its reverse sequence CRAC in the inner one. Their simultaneous presence within the same transmembrane domain obeys a “mirror code” controlling protein-cholesterol interactions in the outer and inner membrane leaflets. Deciphering this code enabled us to elaborate guidelines for the detection of cholesterol-binding motifs in any membrane protein. Several representative examples of neurotransmitter receptors and ABC transporters with the dual CARC/CRAC motifs are presented. The biological significance and potential clinical applications of the mirror code are discussed.
URI : https://repositorio.uca.edu.ar/handle/123456789/8684
ISSN : 2045-2322 (online)
Disciplina: MEDICINA
DOI: 10.1038/srep21907
Derechos: Acceso abierto
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