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Título : Hypothyroidism-associated immunosuppression involves induction of galectin1-producing regulatory T cells
Autor : Valli, Eduardo 
Dalotto Moreno, Tomás 
Sterle, Helena Andrea 
Méndez Huergo, Santiago P. 
Paulazo, Maria Alejandra 
García, Silvia I. 
Pirola, Carlos J. 
Klecha, Alicia Juana 
Rabinovich, Gabriel A. 
Cremaschi, Graciela A. 
Fecha de publicación : 2023
Editorial : Wiley
Cita : Valli, E. et al. Hypothyroidism-associated immunosuppression involves induction of galectin1-producing regulatory T cells [en línea]. Postprint de artículo publicado en The FASEB Journal. 2023, 37 (4). doi:10.1096/fj.202200884R. Disponible en:
Resumen : Abstract: Hypothyroidism exerts deleterious effects on immunity, but the precise role of the hypothalamicpituitary-thyroid (HPT) axis in immunoregulatory and tolerogenic programs is barely understood. Here we investigated the mechanisms underlying hypothyroid-related immunosuppression by examining the regulatory role of components of the HPT axis. We first analyzed lymphocyte activity in mice overexpressing the TRH gene (Tg-Trh). T cells from TgTrh showed increased proliferation than wild type (WT) euthyroid mice in response to polyclonal activation. The release of Th1 proinflammatory cytokines was also increased in TgTrh, and TSH levels correlated with T cell proliferation. To gain further mechanistic insights into hypothyroidism-related immunosuppression, we evaluated T cell subpopulations in lymphoid tissues of hypothyroid and control mice. No differences were observed in CD3/CD19 or CD4/CD8 ratios between these strains. However, the frequency of regulatory T cells (Tregs) was significantly increased in hypothyroid mice, and not in Tg-Trh mice. Accordingly, in vitro Tregs differentiation was more pronounced in naïve T cells isolated from hypothyroid mice. Since Tregs overexpress galectin-1 (Gal-1) and mice lacking this lectin (Lgals1-/-) show reduced Treg function, we investigated the involvement of this immunoregulatory lectin in the control of Tregs in settings of hypothyroidism. Increased T lymphocyte reactivity and reduced frequency of Tregs were found in hypothyroid Lgals1-/- mice when compared to hypothyroid WT animals. This effect was rescued by addition of recombinant Gal-1. Finally, increased expression of Gal-1 was found in Tregs purified from hypothyroid WT mice compared with their euthyroid counterpart. Thus, a substantial increase in the frequency and activity of Gal-1-expressing Tregs underlies immunosuppression associated with hypothyroid conditions, with critical implications in immunopathology, metabolic disorders, and cancer.
ISSN : 1530-6860 (online)
Disciplina: MEDICINA
DOI: 10.1096/fj.202200884R
Derechos: Acceso abierto
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