Please use this identifier to cite or link to this item: https://repositorio.uca.edu.ar/handle/123456789/15390
Título : Cysteine oxidation promotes dimerization/oligomerization of circadian protein period
Autor : Baidanoff, Fernando Martín 
Trebucq, Laura Lucía 
Plano, Santiago Andrés 
Eaton, Phillip 
Golombek, Diego A. 
Chiesa, Juan José 
Palabras clave : REDOXRELOJ CIRCADIANOS-NITROSACIÓNPER2
Fecha de publicación : 2022
Editorial : Multidisciplinary Digital Publishing Institute
Cita : Baidanoff, F. M. et al. Cysteine oxidation promotes dimerization/oligomerization of circadian protein period [en línea]. Biomolecules. 2022, 12 (7). doi: 10.3390/biom12070892. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/15390
Resumen : Abstract: The molecular circadian clock is based on a transcriptional/translational feedback loop in which the stability and half-life of circadian proteins is of importance. Cysteine residues of proteins are subject to several redox reactions leading to S-thiolation and disulfide bond formation, altering protein stability and function. In this work, the ability of the circadian protein period 2 (PER2) to undergo oxidation of cysteine thiols was investigated in HEK-293T cells. PER2 includes accessible cysteines susceptible to oxidation by nitroso cysteine (CysNO), altering its stability by decreasing its monomer form and subsequently increasing PER2 homodimers and multimers. These changes were reversed by treatment with 2-mercaptoethanol and partially mimicked by hydrogen peroxide. These results suggest that cysteine oxidation can prompt PER2 homodimer and multimer formation in vitro, likely by S-nitrosation and disulphide bond formation. These kinds of post-translational modifications of PER2 could be part of the redox regulation of the molecular circadian clock.
URI : https://repositorio.uca.edu.ar/handle/123456789/15390
ISSN : 2218-273X (oniline)
Disciplina: MEDICINA
DOI: 10.3390/biom12070892
Derechos: Acceso abierto
Appears in Collections:Artículos

Files in This Item:
File Description SizeFormat
cysteine-oxidation-promotes-dimerization.pdf671,62 kBAdobe PDFThumbnail
View/Open
Show full item record

Page view(s)

55
checked on Apr 13, 2024

Download(s)

48
checked on Apr 13, 2024

Google ScholarTM

Check


Altmetric


This item is licensed under a Creative Commons License Creative Commons