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Título : Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
Autor : Kamerbeek, Constanza B. 
Mateos, Melina V. 
Vallés, Ana Sofía 
Pediconi, María F. 
Barrantes, Francisco José 
Borroni, María Virginia 
Fecha de publicación : 2016
Editorial : Elsevier
Cita : Kamerbeek, C.B. Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor [en línea]. The International Journal of Biochemistry & Cell Biology. 2016, 74 doi:10.1016/j.biocel.2016.02.010 Disponible en:
Resumen : Abstract: Diacylglycerol (DAG), a second messenger involved in different cell signaling cascades, activates protein kinase C (PKC) and D (PKD), among other kinases. The present work analyzes the effects resulting from the alteration of DAG levels on neuronal and muscle nicotinic acetylcholine receptor (AChR) distribution. We employ CHO-K1/A5 cells, expressing adult muscle-type AChR in a stable manner, and hippocampal neurons, which endogenously express various subtypes of neuronal AChR. CHO-K1/A5 cells treated with dioctanoylglycerol (DOG) for different periods showed augmented AChR cell surface levels at short incubation times (30min-4h) whereas at longer times (18h) the AChR was shifted to intracellular compartments. Similarly, in cultured hippocampal neurons surface AChR levels increased as a result of DOG incubation for 4h. Inhibition of endogenous DAG catabolism produced changes in AChR distribution similar to those induced by DOG treatment. Specific enzyme inhibitors and Western blot assays revealed that DAGs exert their effect on AChR distribution through the modulation of the activity of classical PKC (cPKC), novel PKC (nPKC) and PKD activity.
ISSN : 1357-2725
Disciplina: MEDICINA
DOI: 10.1016/j.biocel.2016.02.010
Derechos: info:eu-repo/semantics/closedAccess
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