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https://repositorio.uca.edu.ar/handle/123456789/14722
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Rodríguez, María Belén | es |
dc.contributor.author | Gambini, Andrés | es |
dc.contributor.author | Clérico, Gabriel José | es |
dc.contributor.author | Ynsaurralde Rivolta, Amada Eugenia | es |
dc.contributor.author | Briski, Olinda | es |
dc.contributor.author | Largel, Hernan | es |
dc.contributor.author | Sansiñena, Marina Julia | es |
dc.contributor.author | Salamone, Daniel F. | es |
dc.date.accessioned | 2022-08-22T13:57:04Z | - |
dc.date.available | 2022-08-22T13:57:04Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Rodríguez, M.B., et al. Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection [en línea]. Reproduction, Fertility and Development. 2019, 31(12) doi:10.1071/RD19248 Disponible en: | es |
dc.identifier.issn | 1031-3613 | - |
dc.identifier.issn | 1448-5990 (online) | - |
dc.identifier.uri | https://repositorio.uca.edu.ar/handle/123456789/14722 | - |
dc.description.abstract | Abstract: Assisted reproduction techniques (ARTs) have become widespread in the equine breeding industry. In particular, the combination of oocyte recovery from live mares followed by IVM and intracytoplasmic sperm injection (ICSI) has increased markedly among the ARTs used with valuable or low-fertility animals. There is currently no consensus among research groups regarding the optimal oocyte maturation period to produce high-quality embryos. In this study, we report the maturation dynamics of equine oocytes at different time points, from 20 to 40 h (Experiment 1). In addition, in Experiment 2, equine ICSI blastocysts were produced from oocytes that exhibited early (up to 24 h) or late (28–30 h) extrusion of the first polar body (PB). Blastocyst rates and diameter were recorded and embryo quality was assessed by analysing the number of apoptotic cells and Yes-associated protein 1 (YAP1) expression. By 20 h of IVM, 42% of oocytes were mature, and the remaining oocytes matured within the next 17 h of IVM. Although no differences were found in cell apoptosis or the number of YAP1-positive cells between groups exhibiting early and late PB extrusion, embryos from the early group (Group I) exhibited an improved total cell number and blastocyst rate compared to embryos from the late group (Group II) (18.60% vs 10.17% respectively). | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | CSIRO Publishing | es |
dc.rights | info:eu-repo/semantics/closedAccess | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.source | Reproduction, Fertility and Development. 2019, 31(12) | es |
dc.subject | CABALLOS | es |
dc.subject | BLASTOCITOS | es |
dc.subject | EMBRIONES | es |
dc.title | Time of first polar body extrusion affects the developmental competence of equine oocytes after intracytoplasmic sperm injection | es |
dc.type | Artículo | es |
dc.identifier.doi | 10.1071/RD19248 | - |
dc.identifier.pmid | 31747534 | - |
uca.disciplina | PRODUCCION AGROPECUARIA | es |
uca.issnrd | 1 | es |
uca.affiliation | Fil: Rodríguez, María Belén. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal; Argentina | es |
uca.affiliation | Fil: Rodríguez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es |
uca.affiliation | Fil: Gambini, Andrés. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal; Argentina | es |
uca.affiliation | Fil: Gambini, Andrés. Consejo Nacio al de Investigaciones Científicas y Técnicas; Argentina | es |
uca.affiliation | Fil: Clérico, Gabriel José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es |
uca.affiliation | Fil: Clérico, Gabriel José. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Biotecnología y Reproducción Animal; Argentina | es |
uca.affiliation | Fil: Ynsaurralde Rivolta, Amada Eugenia. Instituto Nacional de Tecnología Agropecuaria; Argentina | es |
uca.affiliation | Fil: Briski, Olinda. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal; Argentina | es |
uca.affiliation | Fil: Briski, Olinda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es |
uca.affiliation | Fil: Largel, Hernan. El Palenque Embriones Equine Embryo Transfer Center; Argentina | es |
uca.affiliation | Fil: Sansiñena, Marina Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es |
uca.affiliation | Fil: Sansiñena, Marina Julia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Biotecnología y Reproducción Animal; Argentina | es |
uca.affiliation | Fil: Salamone, Daniel F. Universidad de Buenos Aires. Facultad de Agronomía. Laboratorio de Biotecnología Animal; Argentina | es |
uca.affiliation | Fil: Salamone, Daniel F. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es |
uca.version | publishedVersion | es |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.grantfulltext | mixedopen | - |
crisitem.author.dept | Facultad de Ingeniería y Ciencias Agrarias | - |
crisitem.author.parentorg | Pontificia Universidad Católica Argentina | - |
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