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Título : Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
Autor : Mosqueira, Alejo 
Camino, Pablo A. 
Barrantes, Francisco José 
Fecha de publicación : 2018
Editorial : Nature Research
Cita : Mosqueira, A., Camino, P. A., Barranjes, F. J. Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability [en línea]. Scientific Reports. 2018, 8. doi:10.1038/s41598-018-30384-y. Disponible en:
Resumen : Abstract: Translational motion of neurotransmitter receptors is key for determining receptor number at the synapse and hence, synaptic efficacy. We combine live-cell STORM superresolution microscopy of nicotinic acetylcholine receptor (nAChR) with single-particle tracking, mean-squared displacement (MSD), turning angle, ergodicity, and clustering analyses to characterize the lateral motion of individual molecules and their collective behaviour. nAChR diffusion is highly heterogeneous: subdiffusive, Brownian and, less frequently, superdiffusive. At the single-track level, free walks are transiently interrupted by ms-long confinement sojourns occurring in nanodomains of ~36 nm radius. Cholesterol modulates the time and the area spent in confinement. Turning angle analysis reveals anticorrelated steps with time-lag dependence, in good agreement with the permeable fence model. At the ensemble level, nanocluster assembly occurs in second-long bursts separated by periods of cluster disassembly. Thus, millisecond-long confinement sojourns and second-long reversible nanoclustering with similar cholesterol sensitivities affect all trajectories; the proportion of the two regimes determines the resulting macroscopic motional mode and breadth of heterogeneity in the ensemble population.
ISSN : 2045-2322 (online)
Disciplina: MEDICINA
DOI: 10.1038/s41598-018-30384-y
Derechos: Acceso Abierto
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