Please use this identifier to cite or link to this item: https://repositorio.uca.edu.ar/handle/123456789/14600
Título : Enriched environment and visual stimuli protect the retinal pigment epithelium and photoreceptors in a mouse model of non-exudative age-related macular degeneration
Autor : Dieguez, Hernán H. 
Calanni, Juan S. 
Romeo, Horacio 
Alaimo, Agustina 
González Fleitas, María F. 
Iaquinandi, Agustina 
Chianelli, Mónica S. 
Keller Sarmiento, María I. 
Sande, Pablo H. 
Rosenstein, Ruth E. 
Dorfman, Damián 
Palabras clave : DEGENERACIÓN MACULARFISIOPATOLOGÍACÉLULAS FOTORRECEPTORASEPITELIO PIGMENTARIO DE LA RETINAOFTALMOLOGIAFACTORES DE EDADCEGUERAADULTOS
Fecha de publicación : 2021
Editorial : Nature Research
Cita : Dieguez, H. H. et al. Enriched environment and visual stimuli protect the retinal pigment epithelium and photoreceptors in a mouse model of non-exudative age-related macular degeneration [en línea]. Cell Death & Disease. 2021, 12 (1128). doi: 10.1038/s41419-021-04412-1. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14600
Resumen : Abstract: Non-exudative age-related macular degeneration (NE-AMD), the main cause of blindness in people above 50 years old, lacks effective treatments at the moment. We have developed a new NE-AMD model through unilateral superior cervical ganglionectomy (SCGx), which elicits the disease main features in C57Bl/6J mice. The involvement of oxidative stress in the damage induced by NE-AMD to the retinal pigment epithelium (RPE) and outer retina has been strongly supported by evidence. We analysed the effect of enriched environment (EE) and visual stimulation (VS) in the RPE/outer retina damage within experimental NE-AMD. Exposure to EE starting 48 h post-SCGx, which had no effect on the choriocapillaris ubiquitous thickness increase, protected visual functions, prevented the thickness increase of the Bruch's membrane, and the loss of the melanin of the RPE, number of melanosomes, and retinoid isomerohydrolase (RPE65) immunoreactivity, as well as the ultrastructural damage of the RPE and photoreceptors, exclusively circumscribed to the central temporal (but not nasal) region, induced by experimental NE-AMD. EE also prevented the increase in outer retina/RPE oxidative stress markers and decrease in mitochondrial mass at 6 weeks post-SCGx. Moreover, EE increased RPE and retinal brain-derived neurotrophic factor (BDNF) levels, particularly in Müller cells. When EE exposure was delayed (dEE), starting at 4 weeks post-SCGx, it restored visual functions, reversed the RPE melanin content and RPE65-immunoreactivity decrease. Exposing animals to VS protected visual functions and prevented the decrease in RPE melanin content and RPE65 immunoreactivity. These findings suggest that EE housing and VS could become an NE-AMD promising therapeutic strategy.
URI : https://repositorio.uca.edu.ar/handle/123456789/14600
ISSN : 2041-4889 (online)
Disciplina: MEDICINA
DOI: 10.1038/s41419-021-04412-1
Derechos: Acceso abierto
Appears in Collections:Artículos

Files in This Item:
File Description SizeFormat
enriched-environment-visual-stimuli.pdf7,08 MBAdobe PDFThumbnail
View/Open
Show full item record

Page view(s)

13
checked on Aug 9, 2022

Download(s)

1
checked on Aug 9, 2022

Google ScholarTM

Check


Altmetric


This item is licensed under a Creative Commons License Creative Commons