Please use this identifier to cite or link to this item:
Título : Identification and characterization of human PEIG-1/GPRC5A as a 12-Otetradecanoyl phorbol-13-acetate (TPA) and PKC-induced gene
Autor : Mori, Consuelo 
Valdivieso, Ángel Gabriel 
Clauzure, Mariángeles 
Massip Copiz, María M. 
Aguilar, María de los Angeles 
Cafferata, Eduardo G.A. 
Santa Coloma, Tomás Antonio 
Palabras clave : GPRC5APEIG-1RAI3RAID-1TIG1TPA
Fecha de publicación : 2020
Editorial : Elsevier
Cita : Mori, C. et al. Identification and characterization of human PEIG-1/GPRC5A as a 12-Otetradecanoyl phorbol-13-acetate (TPA) and PKC-induced gene [en línea]. Archives of Biochemistry and Biophysics. 2020, 687 (108375). doi: 10.1016/ Disponible en:
Resumen : Abstract: Homo sapiens orphan G protein-coupling receptor PEIG-1 was first cloned and characterized by applying differential display to T84 colonic carcinoma cells incubated in the presence of phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) (GenBank AF506289.1). Later, Lotan's laboratory found the same gene product in response to retinoic acid analogues, naming it with the symbol RAIG1. Now the official HGNC symbol is GPRC5A. Here, we report the extension of its original cDNA fragment towards the 5' and 3' end. In addition, we show that TPA (100 ng/ml, 162 nM) strongly stimulated GPRC5A mRNA in T84 colonic carcinoma cells, with maximal expression at 4 h and 100 ng/ml (162 nM). Western blots showed several bands between 35 and 50 kDa, responding to TPA stimulation. Confocal microscopy confirmed its TPA upregulation and the location in the plasma membrane. The PKC inhibitor Gö 6983 (10 μM), and the Ca2+ chelator BAPTA-AM (150 μM), strongly inhibited its TPA induced upregulation. The PKA inhibitor H-89 (10 μM), and the MEK1/2 inhibitor U0126 (10 μM), also produced a significant reduction in the TPA response (~50%). The SGK1 inhibitor GSK650394 stimulated GPRC5A basal levels at low doses and inhibit its TPA-induced expression at concentrations ≥10 μM. The IL-1β autocrine loop and downstream signalling did not affect its expression. In conclusion, RAIG1/RAI3/GPRC5A corresponds to the originally reported PEIG-1/TIG1; the inhibition observed in the presence of Gö 6983, BAPTA and U0126, suggests that its TPA-induced upregulation is mediated through a PKC/Ca2+ →MEK1/2 signalling axis. PKA and SGK1 kinases are also involved in its TPA-induced upregulation.
ISSN : 0003-9861
1096-0384 (online)
Disciplina: MEDICINA
DOI: 10.1016/
Derechos: Acceso restringido
Appears in Collections:Artículos

Files in This Item:
File Description SizeFormat Existent users please Login
identification-characterization-human-peig-1.pdf3,31 MBAdobe PDF???    Request a copy
Show full item record

Page view(s)

checked on Apr 20, 2024


checked on Apr 20, 2024

Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons