Please use this identifier to cite or link to this item: https://repositorio.uca.edu.ar/handle/123456789/10171
Título : A penicillin derivative exerts an anti-metastatic activity in melanoma cells through the downregulation of Integrin αvβ3 and wnt/β-catenin pathway
Autor : Barrionuevo, Elizabeth 
Cayrol, María Florencia 
Cremaschi, Graciela A. 
Cornier, Patricia G. 
Boggián, Dora B. 
Delpiccolo, Carina M. L. 
Mata, Ernesto G. 
Roguin, Leonor P. 
Blank, Viviana C. 
Palabras clave : PENICILINATUMORESMELANOMAMETASTASIS
Fecha de publicación : 2020
Editorial : Frontiers Media
Cita : Barrionuevo, E., et al. A penicillin derivative exerts an anti-metastatic activity in melanoma cells through the downregulation of Integrin αvβ3 and wnt/β-catenin pathway [en línea]. Frontiers in Pharmacology. 2020, 11:127. doi:10.3389/fphar.2020.00127 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10171
Resumen : Abstact: The synthetic triazolylpeptidyl penicillin derivative, named TAP7f, has been previously characterized as an effective antitumor agent in vitro and in vivo against B16-F0 melanoma cells. In this study, we investigated the anti-metastatic potential of this compound on highly metastatic murine B16-F10 and human A375 melanoma cells. We found that TAP7f inhibited cell adhesion, migration and invasion in a dose-dependent manner. Additionally, we demonstrated that TAP7f downregulated integrin αvβ3 expression and Wnt/β-catenin pathway, a signaling cascade commonly related to tumor invasion and metastasis. Thus, TAP7f reduced both the enzymatic activity and the expression levels of matrix-metalloproteinases-2 and -9 in a time dependent manner. Moreover, TAP7f inhibited the expression of the transcription factor Snail and the mesenchymal markers vimentin, and N-cadherin, and up-regulated the expression of the epithelial marker E-cadherin, suggesting that the penicillin derivative affects epithelial-mesenchymal transition. Results obtained in vitro were supported by those obtained in a B16-F10-bearing mice metastatic model, that showed a significant TAP7f inhibition of lung metastasis. These findings suggest the potential of TAP7f as a chemotherapeutic agent for the treatment of metastatic melanoma.
URI : https://repositorio.uca.edu.ar/handle/123456789/10171
ISSN : 1663-9812
Disciplina: MEDICINA
DOI: 10.3389/fphar.2020.00127
Derechos: Acceso abierto
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