Please use this identifier to cite or link to this item:
|Título :||A penicillin derivative exerts an anti-metastatic activity in melanoma cells through the downregulation of Integrin αvβ3 and wnt/β-catenin pathway||Autor :||Barrionuevo, Elizabeth
Cayrol, María Florencia
Cremaschi, Graciela A.
Cornier, Patricia G.
Boggián, Dora B.
Delpiccolo, Carina M. L.
Mata, Ernesto G.
Roguin, Leonor P.
Blank, Viviana C.
|Palabras clave :||PENICILINA; TUMORES; MELANOMA; METASTASIS||Fecha de publicación :||2020||Editorial :||Frontiers Media||Cita :||Barrionuevo, E., et al. A penicillin derivative exerts an anti-metastatic activity in melanoma cells through the downregulation of Integrin αvβ3 and wnt/β-catenin pathway [en línea]. Frontiers in Pharmacology. 2020, 11:127. doi:10.3389/fphar.2020.00127 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/10171||Resumen :||Abstact: The synthetic triazolylpeptidyl penicillin derivative, named TAP7f, has been previously characterized as an effective antitumor agent in vitro and in vivo against B16-F0 melanoma cells. In this study, we investigated the anti-metastatic potential of this compound on highly metastatic murine B16-F10 and human A375 melanoma cells. We found that TAP7f inhibited cell adhesion, migration and invasion in a dose-dependent manner. Additionally, we demonstrated that TAP7f downregulated integrin αvβ3 expression and Wnt/β-catenin pathway, a signaling cascade commonly related to tumor invasion and metastasis. Thus, TAP7f reduced both the enzymatic activity and the expression levels of matrix-metalloproteinases-2 and -9 in a time dependent manner. Moreover, TAP7f inhibited the expression of the transcription factor Snail and the mesenchymal markers vimentin, and N-cadherin, and up-regulated the expression of the epithelial marker E-cadherin, suggesting that the penicillin derivative affects epithelial-mesenchymal transition. Results obtained in vitro were supported by those obtained in a B16-F10-bearing mice metastatic model, that showed a significant TAP7f inhibition of lung metastasis. These findings suggest the potential of TAP7f as a chemotherapeutic agent for the treatment of metastatic melanoma.||URI :||https://repositorio.uca.edu.ar/handle/123456789/10171||ISSN :||1663-9812||Disciplina:||MEDICINA||DOI:||10.3389/fphar.2020.00127||Derechos:||Acceso abierto|
|Appears in Collections:||Artículos|
Show full item record
Files in This Item:
|penicillin-derivative-exerts-anti-metastatic.pdf||6,59 MB||Adobe PDF|
checked on Mar 5, 2021
checked on Mar 5, 2021
This item is licensed under a Creative Commons License