Por favor, use este identificador para citar o enlazar este ítem:
https://repositorio.uca.edu.ar/handle/123456789/9867
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Tomilin, Viktor N. | es |
dc.contributor.author | Mamenko, Mykola | es |
dc.contributor.author | Zaika, Oleg | es |
dc.contributor.author | Ren, Guohui | es |
dc.contributor.author | Marrelli, Sean P. | es |
dc.contributor.author | Birnbaumer, Lutz | es |
dc.contributor.author | Pochynyuk, Oleh | es |
dc.date.accessioned | 2020-05-05T18:39:40Z | - |
dc.date.available | 2020-05-05T18:39:40Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Tomilin, V.N. et al. TRPC3 determines osmosensitive [Ca2+]i signaling in the collecting duct and contributes to urinary concentration [en línea]. PLoS ONE. 2019, 14(2). doi:10.1371/journal.pone.0226381 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9867 | es |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://repositorio.uca.edu.ar/handle/123456789/9867 | - |
dc.description.abstract | Abstract: It is well-established that the kidney collecting duct (CD) plays a central role in regulation of systemic water homeostasis. Aquaporin 2 (AQP2)-dependent water reabsorption in the CD critically depends on the arginine vasopressin (AVP) antidiuretic input and the presence of a favorable osmotic gradient at the apical plasma membrane with tubular lumen being hypotonic compared to the cytosol. This osmotic difference creates a mechanical force leading to an increase in [Ca2+]i in CD cells. The significance of the osmosensitive [Ca2+]i signaling for renal water transport and urinary concentration remain unknown. To examine molecular mechanism and physiological relevance of osmosensitivity in the CD, we implemented simultaneous direct measurements of [Ca2+]i dynamics and the rate of cell swelling as a readout of the AQP2-dependent water reabsorption in freshly isolated split-opened CDs of wild type and genetically manipulated animals and combined this with immunofluorescent detection of AVP-induced AQP2 trafficking and assessment of systemic water balance. We identified the critical role of the Ca2+-permeable TRPC3 channel in osmosensitivity and water permeability in the CD. We further demonstrated that TRPC3 -/- mice exhibit impaired urinary concentration, larger urinary volume and a greater weight loss in response to water deprivation despite increased AVP levels and AQP2 abundance. TRPC3 deletion interfered with AQP2 translocation to the plasma membrane in response to water deprivation. In summary, we provide compelling multicomponent evidence in support of a critical contribution of TRPC3 in the CD for osmosensitivity and renal water handling. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.rights | Acceso abierto | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.source | PLoS ONE. 2019, 14(2) | es |
dc.subject | CALCIO | es |
dc.subject | SEÑALIZACION INTRACELULAR | es |
dc.subject | RIÑON | es |
dc.subject | PRESION OSMOTICA | es |
dc.subject | TRPC3 | es |
dc.subject | ACUAPORINAS | es |
dc.title | TRPC3 determines osmosensitive [Ca2+]i signaling in the collecting duct and contributes to urinary concentration | es |
dc.type | Artículo | es |
dc.identifier.doi | 10.1371/journal.pone.0226381 | - |
dc.identifier.pmid | 31851715 | - |
uca.disciplina | MEDICINA | es |
uca.issnrd | 1 | es |
uca.affiliation | Fil: Tomilin, Viktor N. University of Texas. Health Science Center at Houston. Department of Integrative Biology and Pharmacology; Estados Unidos | es |
uca.affiliation | Fil: Mamenko, Mykola. Augusta University. Medical College of Georgia. Department of Physiology; Estados Unidos | es |
uca.affiliation | Fil: Zaika, Oleg. University of Texas. Health Science Center at Houston. Department of Integrative Biology and Pharmacology; Estados Unidos | es |
uca.affiliation | Fil: Ren, Guohui. University of Texas. Health Science Center at Houston. Department of Integrative Biology and Pharmacology; Estados Unidos | es |
uca.affiliation | Fil: Marrelli, Sean P. University of Texas. Health Science Center at Houston. Department of Neurology; Estados Unidos | es |
uca.affiliation | Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina | es |
uca.affiliation | Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos | es |
uca.affiliation | Fil: Pochynyuk, Oleh. University of Texas. Health Science Center at Houston. Department of Integrative Biology and Pharmacology; Estados Unidos | es |
uca.version | publishedVersion | es |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
crisitem.author.dept | Instituto de Investigaciones Biomédicas - BIOMED | - |
crisitem.author.dept | Laboratorio de Función y Farmacología de Canales Iónicos | - |
crisitem.author.dept | Consejo Nacional de Investigaciones Científicas y Técnicas | - |
crisitem.author.dept | Facultad de Ciencias Médicas | - |
crisitem.author.orcid | 0000-0002-0775-8661 | - |
crisitem.author.orcid | 0000-0002-5397-6897 | - |
crisitem.author.parentorg | Facultad de Ciencias Médicas | - |
crisitem.author.parentorg | Instituto de Investigaciones Biomédicas - BIOMED | - |
crisitem.author.parentorg | Pontificia Universidad Católica Argentina | - |
Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
trpc3-determines-osmosensitive.pdf | 3,68 MB | Adobe PDF | Visualizar/Abrir |
Visualizaciones de página(s)
105
comprobado en 30-abr-2024
Descarga(s)
104
comprobado en 30-abr-2024
Google ScholarTM
Consultar
Altmetric
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons