Please use this identifier to cite or link to this item: https://repositorio.uca.edu.ar/handle/123456789/8778
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dc.contributor.authorClauzure, Mariángeleses
dc.contributor.authorValdivieso, Ángel Gabrieles
dc.contributor.authorMassip Copiz, María Macarenaes
dc.contributor.authorMori, Consueloes
dc.contributor.authorDugour, Andrea V.es
dc.contributor.authorFigueroa, Juan M.es
dc.contributor.authorSanta Coloma, Tomás Antonioes
dc.date.accessioned2019-09-23T15:07:52Z-
dc.date.available2019-09-23T15:07:52Z-
dc.date.issued2017-
dc.identifier.citationClauzure M, Valdivieso ÁG, Massip‐Copiz MM, et al. Intracellular Chloride Concentration Changes Modulate IL-1β Expression and Secretion in Human Bronchial Epithelial Cultured Cells. Journal of Cellular Biochemistry. 2017;118(8):2131-2140. doi:10.1002/jcb.25850 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8778es
dc.identifier.issn0730-2312 (impreso)-
dc.identifier.issn1097-4644 (online)-
dc.identifier.urihttps://repositorio.uca.edu.ar/handle/123456789/8778-
dc.description.abstractAbstract: Cystic fibrosis (CF) is caused by mutations in the CFTR gene, which encodes a cAMP-regulated chloride channel. Several cellular functions are altered in CF cells. However, it is not clear how the CFTR failure induces those alterations. We have found previously several genes differentially expressed in CF cells, including c-Src, MUC1, MTND4, and CISD1 (CFTR-dependent genes). Recently, we also reported the existence of several chloride-dependent genes, among them GLRX5 and RPS27. Here, varying the intracellular chloride concentration [Cl- ]i of IB3-1 CF bronchial epithelial cells, we show that IL-1β mRNA expression and secretion are also under Cl- modulation. The response to Cl- is biphasic, with maximal effects at 75 mM Cl- . The regulation of the IL-1β mRNA expression involves an IL-1β autocrine effect, since in the presence of the IL-1β receptor antagonist IL1RN or anti-IL-1β blocking antibody, the mRNA response to Cl- disappeared. Similar effects were obtained with the JNK inhibitor SP600125, the c-Src inhibitor PP2 and the IKK inhibitor III (BMS-345541). On the other hand, the IL-1β secretion is still modulated by Cl- in the presence of IL-1RN, IL-1β blocking antibody, or cycloheximide, suggesting that Cl- is affecting the IL-1β maturation/secretion, which in turn starts an autocrine positive feedback loop. In conclusion, the Cl- anion acts as a second messenger for CFTR, modulating the IL-1β maturation/secretion. The results also imply that, depending on its intracellular concentration, Cl- could be a pro-inflammatory mediator. J. Cell. Biochem. 118: 2131-2140, 2017. © 2016 Wiley Periodicals, Inc.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherWileyes
dc.rightsAcceso Abierto*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.sourceJournal of Cellular Biochemistry. 2017;118(8):2131-2140es
dc.subjectCELULAS EPITELIALESes
dc.subjectMARCADORES GENETICOSes
dc.subjectFIBROSIS QUISTICAes
dc.subjectINFLAMACIONes
dc.subjectANIONESes
dc.subjectCLORURO INTRACELULARes
dc.titleIntracellular chloride concentration changes modulate IL-1β expression and secretion in human bronchial epithelial cultured cellses
dc.typeArtículoes
dc.identifier.doi10.1002/jcb.25850-
dc.identifier.pmid27996167-
uca.disciplinaMEDICINAes
uca.issnrd1es
uca.affiliationFil: Clauzure, Mariángeles. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Biología Celular y Molecular; Argentinaes
uca.affiliationFil: Clauzure, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes
uca.affiliationFil: Valdivieso, Ángel Gabriel. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Biología Celular y Molecular; Argentinaes
uca.affiliationFil: Valdivieso, Ángel Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes
uca.affiliationFil: Massip Copiz, María Macarena. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Biología Celular y Molecular; Argentinaes
uca.affiliationFil: Massip Copiz, María Macarena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes
uca.affiliationFil: Mori, Consuelo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Biología Celular y Molecular; Argentinaes
uca.affiliationFil: Mori, Consuelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes
uca.affiliationFil: Dugour, Andrea V. Fundación Pablo Cassará; Argentinaes
uca.affiliationFil: Figueroa, Juan M. Fundación Pablo Cassará; Argentinaes
uca.affiliationFil: Santa Coloma, Tomás Antonio. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Biología Celular y Molecular; Argentinaes
uca.affiliationFil: Santa Coloma, Tomás Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes
uca.versionacceptedVersiones
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.languageiso639-1en-
crisitem.author.deptLaboratorio de Biología Celular y Molecular-
crisitem.author.deptInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.deptLaboratorio de Biología Celular y Molecular-
crisitem.author.deptConsejo Nacional de Investigaciones Científicas y Técnicas-
crisitem.author.deptInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.deptLaboratorio de Biología Celular y Molecular-
crisitem.author.deptInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.deptLaboratorio de Biología Celular y Molecular-
crisitem.author.deptConsejo Nacional de Investigaciones Científicas y Técnicas-
crisitem.author.deptInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.deptLaboratorio de Biología Celular y Molecular-
crisitem.author.deptLaboratorio de Nanotecnología-
crisitem.author.deptConsejo Nacional de Investigaciones Científicas y Técnicas-
crisitem.author.orcid0000-0002-3266-1095-
crisitem.author.parentorgInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.parentorgFacultad de Ciencias Médicas-
crisitem.author.parentorgInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.parentorgFacultad de Ciencias Médicas-
crisitem.author.parentorgInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.parentorgFacultad de Ciencias Médicas-
crisitem.author.parentorgInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.parentorgFacultad de Ciencias Médicas-
crisitem.author.parentorgInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.parentorgInstituto de Investigaciones Biomédicas - BIOMED-
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