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dc.contributor.authorKamerbeek, Constanza B.es
dc.contributor.authorBorroni, María Virginiaes
dc.contributor.authorPediconi, María F.es
dc.contributor.authorSato, Satoshi B.es
dc.contributor.authorKobayashi, Toshihidees
dc.contributor.authorBarrantes, Francisco Josées
dc.date.accessioned2019-09-17T15:33:41Z-
dc.date.available2019-09-17T15:33:41Z-
dc.date.issued2013-
dc.identifier.citationKamerbeek CB, Borroni V, Pediconi MF, Sato SB, Kobayashi T, Barrantes FJ. Antibody-induced acetylcholine receptor clusters inhabit liquid-ordered and liquid-disordered domains. Biophys J. 2013;105(7):1601–1611. doi:10.1016/j.bpj.2013.08.039. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8752es
dc.identifier.issn0006-3495 (impreso)-
dc.identifier.issn1542-0086 (online)-
dc.identifier.urihttps://repositorio.uca.edu.ar/handle/123456789/8752-
dc.description.abstractAbstract: The distribution of nicotinic acetylcholine receptor (AChR) clusters at the cell membrane was studied in CHO-K1/A5 cells using fluorescence microscopy. Di-4-ANEPPDHQ, a fluorescent probe that differentiates between liquid-ordered (Lo) and liquid-disordered (Ld) phases in model membranes, was used in combination with monoclonal anti-AChR antibody labeling of live cells, which induces AChR clustering. The so-called generalized polarization (GP) of di-4-ANEPPDHQ was measured in regions of the cell-surface membrane associated with or devoid of antibody-induced AChR clusters, respectively. AChR clusters were almost equally distributed between Lo and Ld domains, independently of receptor surface levels and agonist (carbamoylcholine and nicotine) or antagonist (α-bungarotoxin) binding. Cholesterol depletion diminished the cell membrane mean di-4-ANEPPDHQ GP and the number of AChR clusters associated with Ld membrane domains increased concomitantly. Depolymerization of the filamentous actin cytoskeleton by Latrunculin A had the opposite effect, with more AChR clusters associated with Lo domains. AChR internalized via small vesicles having lower GP and lower cholesterol content than the surface membrane. Upon cholesterol depletion, only 12% of the AChR-containing vesicles costained with the fluorescent cholesterol analog fPEG-cholesterol, i.e., AChR endocytosis was essentially dissociated from that of cholesterol. In conclusion, the distribution of AChR submicron-sized clusters at the cell membrane appears to be regulated by cholesterol content and cytoskeleton integrity.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherElsevier B.V.es
dc.rightsAcceso abierto*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.sourceBiophysical Journal Vol. 105, N° 7, 2013es
dc.subjectMEDICINAes
dc.subjectRECEPTORESes
dc.subjectMEMBRANAS CELULARESes
dc.subjectCOLESTEROLes
dc.subjectANTICUERPOSes
dc.titleAntibody-induced acetylcholine receptor clusters inhabit liquid-ordered and liquid-disordered domainses
dc.typeArtículoes
dc.identifier.doi10.1016/j.bpj.2013.08.039-
dc.identifier.pmid24094401-
uca.disciplinaMEDICINAes
uca.issnrd1es
uca.affiliationFil: Kamerbeek, Constanza B. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentinaes
uca.affiliationFil: Borroni, María Virginia. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentinaes
uca.affiliationFil: Pediconi, María F. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentinaes
uca.affiliationFil: Sato, Satoshi B. Lipid Biology Laboratory, RIKEN; Japónes
uca.affiliationFil: Kobayashi, Toshihide. Lipid Biology Laboratory, RIKEN; Japónes
uca.affiliationFil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentinaes
uca.affiliationFil: Barrantes Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentinaes
uca.affiliationFil: Barrantes, Francisco José. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentinaes
uca.versionpublishedVersiones
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.languageiso639-1en-
crisitem.author.deptInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.deptLaboratorio de Neurobiología Molecular-
crisitem.author.deptFacultad de Ciencias Médicas-
crisitem.author.orcid0000-0002-4745-681X-
crisitem.author.parentorgFacultad de Ciencias Médicas-
crisitem.author.parentorgInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.parentorgPontificia Universidad Católica Argentina-
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