Por favor, use este identificador para citar o enlazar este ítem:
https://repositorio.uca.edu.ar/handle/123456789/14595
Título : | Measurement of cystic fibrosis transmembrane conductance regulator activity using fluorescence spectrophotometry | Autor : | Valdivieso, Ángel Gabriel Marín, María C. Clauzure, Mariángeles Santa Coloma, Tomás Antonio |
Palabras clave : | FIBROSIS QUÍSTICA; CANAL DE CLORURO; ESPECTROFOTOMETRÍA; REGULADOR DE CONDUCTANCIA TRANSMEMBRANA DE LA FIBROSIS QUÍSTICA; FLUORESCENCIA | Fecha de publicación : | 2011 | Editorial : | Elsevier | Cita : | Valdivieso, Á. G. Measurement of cystic fibrosis transmembrane conductance regulator activity using fluorescence spectrophotometry [en línea]. Analytical Biochemistry Bioquímica Analítica. 2011, 418 (2). doi: 10.1016/j.ab.2011.07.029. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14595 | Resumen : | Abstract: Cystic fibrosis (CF) is a frequent autosomal recessive disease caused by mutations that impair the CF transmembrane conductance regulator (CFTR) protein function. CFTR is a chloride channel activated by cyclic AMP (cAMP) via protein kinase A (PKA) and ATP hydrolysis. We describe here a method to measure CFTR activity in a monolayer of cultured cells using a fluorescence spectrophotometer and the chloridesensitive probe 6-methoxy-N-(3-sulfopropyl)quinolinium (SPQ). Modifying a slice holder, the spectrophotometer quartz cuvette was converted in a perfusion chamber, allowing measurement of CFTR activity in real time, in a monolayer of T84 colon carcinoma cells. The SPQ Stern–Volmer constant (KCl ) for chloride in water solution was 115.0 ± 2.8 M1 , whereas the intracellular KCl was 17.8 ± 0.8 M1 , for T84 cells. A functional analysis was performed by measuring CFTR activity in T84 cells. The CFTR transport inhibitors CFTR(inh)-172 (5 lM) and glibenclamide (100 lM) showed a significant reduction (P < 0.05) in CFTR activity. This simple method allows measuring CFTR activity in a very simple, reproducible, and sensitive way. | URI : | https://repositorio.uca.edu.ar/handle/123456789/14595 | ISSN : | 0003-2697 1096-0309 (online) |
Disciplina: | MEDICINA | DOI: | 10.1016/j.ab.2011.07.029 | Derechos: | Acceso restringido |
Aparece en las colecciones: | Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | Usuarios registrados haga click en: Login |
---|---|---|---|---|
measurement-cystic-fibrosis-transmembrane.pdf | 615,9 kB | Adobe PDF | Acceso restringido |
Visualizaciones de página(s)
61
comprobado en 27-abr-2024
Descarga(s)
14
comprobado en 27-abr-2024
Google ScholarTM
Consultar
Altmetric
Este ítem está sujeto a una licencia Creative Commons Licencia Creative Commons