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dc.contributor.authorMosqueira, Alejoes
dc.contributor.authorCamino, Pablo A.es
dc.contributor.authorBarrantes, Francisco Josées
dc.date.accessioned2022-06-22T17:25:01Z-
dc.date.available2022-06-22T17:25:01Z-
dc.date.issued2020-
dc.identifier.citationMosqueira, A., Camino, P.A., Barrantes, F.J. Antibody-induced crosslinking and cholesterol-sensitive, anomalous diffusion of nicotinic acetylcholine receptors [en línea]. Journal of Neurochemistry. 2020, 152(6) doi:10.1111/jnc.14905 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14228es
dc.identifier.issn1471-4159-
dc.identifier.urihttps://repositorio.uca.edu.ar/handle/123456789/14228-
dc.description.abstractAbstract: Synaptic strength depends on the number of cell-surface neurotransmitter receptors in dynamic equilibrium with intracellular pools. Dysregulation of this homeostatic balance occurs, for example in myasthenia gravis, an autoimmune disease characterized by a decrease in the number of postsynaptic nicotinic acetylcholine receptors (nAChRs). Monoclonal antibody mAb35 mimics this effect. Here we use STORM nanoscopy to characterize the individual and ensemble dynamics of monoclonal antibody-crosslinked receptors in the clonal cell line CHO-K1/A5, which robustly expresses adult muscle-type nAChRs. Antibody labeling of live cells results in 80% receptor immobilization. The remaining mobile fraction exhibits a heterogeneous combination of Brownian and anomalous diffusion. Single-molecule trajectories exhibit a two-state switching behavior between free Brownian walks and anticorrelated walks within confinement areas. The latter act as permeable fences (~34 nm radius, ~400 ms lifetime). Dynamic clustering, trapping, and immobilization also occur in larger nanocluster zones (120-180 nm radius) with longer lifetimes (11 ± 1 s), in a strongly cholesterol-sensitive manner. Cholesterol depletion increases the size of the clustering phenomenon; cholesterol enrichment has the opposite effect. The disclosed high proportion of monoclonal antibody-crosslinked immobile receptors, together with their anomalous, cholesterol-sensitive diffusion and clustering, provides new insights into the antibody-enhanced antigenic modulation that leads to physiopathological internalization and degradation of receptors in myasthenia.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherWileyes
dc.rightsAcceso abierto*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.sourceJournal of Neurochemistry. 2020, 152(6)es
dc.subjectMIASTENIA GRAVISes
dc.subjectANTICUERPOS MONOCLONALESes
dc.subjectCOLESTEROLes
dc.titleAntibody-induced crosslinking and cholesterol-sensitive, anomalous diffusion of nicotinic acetylcholine receptorses
dc.typeArtículoes
dc.identifier.doi10.1111/jnc.14905-
dc.identifier.pmid31777963-
uca.disciplinaMEDICINAes
uca.issnrd1es
uca.affiliationFil: Mosqueira, Alejo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentinaes
uca.affiliationFil: Camino, Pablo A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentinaes
uca.affiliationFil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentinaes
uca.versionpublishedVersiones
item.grantfulltextopen-
item.fulltextWith Fulltext-
item.languageiso639-1en-
crisitem.author.deptInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.deptLaboratorio de Neurobiología Molecular-
crisitem.author.deptFacultad de Ciencias Médicas-
crisitem.author.deptInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.deptLaboratorio de Neurobiología Molecular-
crisitem.author.deptFacultad de Ciencias Médicas-
crisitem.author.orcid0000-0002-5693-5302-
crisitem.author.orcid0000-0002-4745-681X-
crisitem.author.parentorgFacultad de Ciencias Médicas-
crisitem.author.parentorgInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.parentorgPontificia Universidad Católica Argentina-
crisitem.author.parentorgFacultad de Ciencias Médicas-
crisitem.author.parentorgInstituto de Investigaciones Biomédicas - BIOMED-
crisitem.author.parentorgPontificia Universidad Católica Argentina-
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