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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Wang, Jue | es |
dc.contributor.author | Hertz, Laura | es |
dc.contributor.author | Ruppenthal, Sandra | es |
dc.contributor.author | El Nemer, Wassim | es |
dc.contributor.author | Connes, Philippe | es |
dc.contributor.author | Goede, Jeroen S. | es |
dc.contributor.author | Bogdanova, Anna | es |
dc.contributor.author | Birnbaumer, Lutz | es |
dc.contributor.author | Kaestner, Lars | es |
dc.date.accessioned | 2021-06-14T16:22:19Z | - |
dc.date.available | 2021-06-14T16:22:19Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Wang, J., et al. Lysophosphatidic acid-activated calcium signaling is elevated in red cells from sickle cell disease patients [en línea]. Cells. 2021, 10 (2), 456. doi:10.3390/cells10020456. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11619 | es |
dc.identifier.issn | 2073-4409 | - |
dc.identifier.uri | https://repositorio.uca.edu.ar/handle/123456789/11619 | - |
dc.description.abstract | Abstract: (1) Background: It is known that sickle cells contain a higher amount of Ca2+ compared to healthy red blood cells (RBCs). The increased Ca2+ is associated with the most severe symptom of sickle cell disease (SCD), the vaso-occlusive crisis (VOC). The Ca2+ entry pathway received the name of Psickle but its molecular identity remains only partly resolved. We aimed to map the involved Ca2+ signaling to provide putative pharmacological targets for treatment. (2) Methods: The main technique applied was Ca2+ imaging of RBCs from healthy donors, SCD patients and a number of transgenic mouse models in comparison to wild-type mice. Life-cell Ca2+ imaging was applied to monitor responses to pharmacological targeting of the elements of signaling cascades. Infection as a trigger of VOC was imitated by stimulation of RBCs with lysophosphatidic acid (LPA). These measurements were complemented with biochemical assays. (3) Results: Ca2+ entry into SCD RBCs in response to LPA stimulation exceeded that of healthy donors. LPA receptor 4 levels were increased in SCD RBCs. Their activation was followed by the activation of Gi protein, which in turn triggered opening of TRPC6 and CaV2.1 channels via a protein kinase C and a MAP kinase pathway, respectively. (4) Conclusions: We found a new Ca2+ signaling cascade that is increased in SCD patients and identified new pharmacological targets that might be promising in addressing the most severe symptom of SCD, the VOC. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.rights | Acceso abierto | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.source | Cells Vol. 10, No. 2, 456, 2021 | es |
dc.subject | ENFERMEDAD DE CELULAS FALCIFORMES | es |
dc.subject | ERITROCITOS | es |
dc.subject | HISTOLOGIA | es |
dc.subject | CALCIO | es |
dc.subject | ANEMIA HEMOLITICA | es |
dc.title | Lysophosphatidic acid-activated calcium signaling is elevated in red cells from sickle cell disease patients | es |
dc.type | Artículo | es |
dc.identifier.doi | https://doi.org/10.3390/cells10020456 | - |
dc.identifier.pmid | 33672679 | - |
uca.disciplina | MEDICINA | es |
uca.issnrd | 1 | es |
uca.affiliation | Fil: Wang, Jue. University of Texas Health Science Center at Tyler. Department of Cellular and Molecular Biology; Estados Unidos | es |
uca.affiliation | Fil: Hertz, Laura. Saarland University. Theoretical Medicine and Biosciences; Alemania | es |
uca.affiliation | Fil: Hertz, Laura. Saarland University. Experimental Physics, Dynamics of Fluids; Alemania | es |
uca.affiliation | Fil: Ruppenthal, Sandra. Saarland University. Experimental Physics, Dynamics of Fluids; Alemania | es |
uca.affiliation | Fil: Ruppenthal, Sandra. Saarland University Hospital. Gynaecology, Obstetrics and Reproductive Medicine; Alemania | es |
uca.affiliation | Fil: El Nemer, Wassim. Aix Marseille Université. Etablissement Français du Sang PACA-Corse; Francia | es |
uca.affiliation | Fil: El Nemer, Wassim. Laboratoire d’Excellence GR-Ex; Francia | es |
uca.affiliation | Fil: Connes, Philippe. Laboratoire d’Excellence GR-Ex; Francia | es |
uca.affiliation | Fil: Connes, Philippe. University Claude Bernard Lyon 1. Vascular Biology and Red Blood Cell Teal. Laboratory LIBM EA7424; Francia | es |
uca.affiliation | Fil: Goede, Jeroen S. Kantonsspital Winterthur. Division of Oncology and Hematology; Suiza | es |
uca.affiliation | Fil: Bogdanova, Anna. University of Zürich. Institute of Veterinary Physiology. Red Blood Cell Research Group; Suiza | es |
uca.affiliation | Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina | es |
uca.affiliation | Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos | es |
uca.affiliation | Fil: Kaestner, Lars. Saarland University. Theoretical Medicine and Biosciences; Alemania | es |
uca.affiliation | Fil: Kaestner, Lars. Saarland University. Experimental Physics, Dynamics of Fluids; Alemania | es |
uca.version | publishedVersion | es |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | Instituto de Investigaciones Biomédicas - BIOMED | - |
crisitem.author.dept | Laboratorio de Función y Farmacología de Canales Iónicos | - |
crisitem.author.dept | Consejo Nacional de Investigaciones Científicas y Técnicas | - |
crisitem.author.dept | Facultad de Ciencias Médicas | - |
crisitem.author.orcid | 0000-0002-0775-8661 | - |
crisitem.author.parentorg | Facultad de Ciencias Médicas | - |
crisitem.author.parentorg | Instituto de Investigaciones Biomédicas - BIOMED | - |
crisitem.author.parentorg | Pontificia Universidad Católica Argentina | - |
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