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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Plano, Santiago Andrés | es |
dc.contributor.author | Alessandro, María Soledad | es |
dc.contributor.author | Trebucq, Laura Lucía | es |
dc.contributor.author | Endo, Shogo | es |
dc.contributor.author | Golombek, Diego A. | es |
dc.contributor.author | Chiesa, Juan José | es |
dc.date.accessioned | 2021-05-13T15:28:58Z | - |
dc.date.available | 2021-05-13T15:28:58Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Plano, S. A., Alessandro, M. S., Trebucq, L. L., Endo, S., Golombek, D. A., Chiesa, J. J. Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock [en línea]. American Society for Neurochemistry. 2021, 13(1-12). Doi: 10.1177/1759091420984920. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11524 | es |
dc.identifier.issn | 1759-0914 (on line) | - |
dc.identifier.uri | https://repositorio.uca.edu.ar/handle/123456789/11524 | - |
dc.description.abstract | Abstract: The mammalian circadian clock at the hypothalamic suprachiasmatic nuclei (SCN) entrains biological rhythms to the 24- h cyclic environment, by encoding light-dark transitions in SCN neurons. Light pulses induce phase shifts in the clock and in circadian rhythms; photic signaling for circadian phase advances involves a nitric oxide (NO)/cyclic guanosine monophosphate (cGMP)/cGMP-dependent protein kinase (PKG) pathway, increasing the expression of Period (Per) genes. Effectors downstream of PKG remain unknown. Here we investigate the role of G-substrate (GS), a PKG substrate, in the hamster SCN. GS and phosphorylated G-substrate (p-GS) were present in a subset of SCN cells. Moreover, GS phosphorylation (p- GS/GS ratio) increased in SCN homogenates after light pulses delivered at circadian time (CT) 18 and intraperitoneal treatment with sildenafil, an inhibitor of phosphodiesterase 5 (a cGMP-specific phosphodiesterase). On the other hand, intracerebroventricular treatment with the PKG inhibitor KT5823, reduced photic phosphorylation of GS to basal levels. Since p-GS could act as a protein phosphatase 2 A (PP2A) inhibitor, we demonstrated physical interaction between p-GS and PP2A in SCN homogenates, and also a light-pulse dependent decrease of PP2A activity. Intracerebroventricular treatment with okadaic acid, a PP2A inhibitor, increased the magnitude of light-induced phase advances of locomotor rhythms. We provide evidence on the physiological phosphorylation of GS as a new downstream effector in the NO/cGMP/PKG photic pathway in the hamster SCN, including its role as a PP2A inhibitor. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | SAGE Publishing | es |
dc.rights | Atribución-NoComercial-CompartirIgual 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/4.0/ | * |
dc.source | American Society for Neurochemistry. 2021, 13(1-12) | es |
dc.subject | NUCLEO SUPRAQUIASMATICO | es |
dc.subject | PROTEíNA FOSFATASA 2A | es |
dc.subject | SISTEMA NERVIOSO CENTRAL | es |
dc.subject | LUZ | es |
dc.subject | RITMO CIRCADIANO | es |
dc.title | Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock | es |
dc.type | Artículo | es |
dc.identifier.doi | 10.1177/1759091420984920 | - |
dc.identifier.pmid | 33430619 | - |
uca.disciplina | MEDICINA | es |
uca.issnrd | 1 | es |
uca.affiliation | Fil: Plano, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es |
uca.affiliation | Fil: Plano, Santiago Andrés. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina | es |
uca.affiliation | Fil: Alessandro, María Soledad. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina | es |
uca.affiliation | Fil: Plano, Santiago Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina | es |
uca.affiliation | Fil: Alessandro, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es |
uca.affiliation | Fil: Trebucq, Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es |
uca.affiliation | Fil: Trebucq, Laura Lucía. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina | es |
uca.affiliation | Fil: Endo, Shogo. Instituto Metropolitano de Gerontología de Tokio; Japón | es |
uca.affiliation | Fil: Golombek, Diego A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es |
uca.affiliation | Fil: Golombek, Diego A. Universidad Nacional de Quilmes. Laboratorio de Cronobiología; Argentina | es |
uca.affiliation | Fil: Chiesa, Juan José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina | es |
uca.affiliation | Fil: Chiesa, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina | es |
uca.version | publishedVersion | es |
item.grantfulltext | open | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | Instituto de Investigaciones Biomédicas - BIOMED | - |
crisitem.author.dept | Laboratorio de Cronofisiología | - |
crisitem.author.dept | Consejo Nacional de Investigaciones Científicas y Técnicas | - |
crisitem.author.dept | Facultad de Ciencias Médicas | - |
crisitem.author.orcid | 0000-0002-7984-8826 | - |
crisitem.author.parentorg | Facultad de Ciencias Médicas | - |
crisitem.author.parentorg | Instituto de Investigaciones Biomédicas - BIOMED | - |
crisitem.author.parentorg | Pontificia Universidad Católica Argentina | - |
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